Our Testing and Validation Methods

OBI uses cutting-edge testing to ensure the safety, efficacy, and molecular precision of all bioactive ingredients and formulations. Our qPCR DNA/RNA analysis enables rapid, cost-effective evaluation of cellular and genetic effects, providing deeper insights into skin aging and product performance. Chemical and physical validation is conducted using FTIR, UV-VIS, HPLC/GC, viscometry, and SPF testing.

Efficacy and safety are further confirmed in collaboration with Therapeutic Peptides Inc., utilizing human cell cultures, ELISA, CyQuant, and other advanced assays, all supported by state-of-the-art on-site laboratories and instrumentation.

Chemical Validation

OBI evaluates the chemical properties of ingredients using
advanced techniques, including:

  • FTIR and UV-VIS Spectroscopy
  • Laser Low Angle Light Scattering
  • SPF UV Trans Spectrometry
  • Multiple HPLC and GC Systems
  • Automated Titration Equipment
  • Molecular Weight Determination
  • Viscometry

Efficacy and Safety

In collaboration with Therapeutic Peptide Inc., OBI evaluates bioactive ingredients by analyzing their effects on gene expression in keratinocytes, fibroblasts, and melanocytes. Results are validated using ELISA, CyQuant, soluble protein assays, and other conventional methods.

Scientist with equipment and science experiments

On-Site Resources Include:

  • Cell and Tissue Laboratory
  • Microbiology Laboratory
  • qPCR Roche Systems (42 and 82 Gene Arrays)
  • Fluorescence and UV/Vis Microplate Readers
  • CyQuant / Rate of Proliferation Enhancement
  • Extracellular Soluble Protein Enhancement
  • Alamar Blue / Cytotoxicity LD50
  • ELISA Kits (Defensin B, UV DNA Damage, Oxidative DNA Damage, etc.)
  • UVA / UVB Lamp Assembly
  • Visible, UV, and Phase Contrast Microscopy