The equipment used to validate ingredient synthesis includes:
FTIR UV-VIS Laser Low Angle Light scattering spectrometers SPF UV Trans Spectrometer Multiple HPLC's, GC's Automated Titration Equipment Molecular Weight determination Viscometry
OBI, in cooperation with TPI, operates a joint program to develop a library of potential bioactive ingredients classified as to their effects on gene expression after addition to keratinocytes, fibroblasts and melanocytes in human cell culture. These results are correlated with conventional ELISA, CyQuant and Soluble Protein Assays. This work is conducted with the following on site resources:
Cell and Tissue Laboratory Microbiology Laboratory qPCR Roche System (Roche) 42 and 82 gene arrays Fluorescence Microplate Readers UV / Vis Microplate Readers CyQuant / Rate of Proliferation Enhancement Extracellular Soluble Protein Enhancement Alamar Blue / Cytotoxicity LD50 Elisa Kits (Defensin B, UVDNA damage, Oxidative DNA damage etc.) UVA / UVB Lamp assembly Visible, UV, Phase Contrast Photo Microscopy
Anti-aging (cell turnover enhancement/ soluble protein production): TPI has conducted qPCR gene array assays for the top 30 ingredients claimed for anti-aging on four cell types (keratinocytes, fibroblasts (37 yoff, 66 yoff), keratinocytes, and melanocytes) as a reference library.
Pigmentation (pigment suppression/ selective pheomelannin production): TPI has conducted qPCR gene array assays on the top 20 ingredients claimed for pigmentation/ anti-oxidant activity on melanocytes as a reference library.
Oxidation / UV DNA Repair: Produce qPCR gene array assays on top 30 ingredients claimed for pigmentation / anti-oxidant activity on four cell types.
